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A Guide to Sample Size for Animal-based Studies Alps Much of what men andUnderstand a foundational area of experimental design with this innovative reference Animal based research is an essential part of basic and preclinical research, but poses a unique set of experimental design challenges. The most important of these are the 3Rs Replacement, Reduction and Refinement the principles comprising the ethical framework for humane animal based studies. However, many researchers have difficulty navigating the design trade offs