Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g Improvement of a synthetic medium

Improvement of a synthetic medium for Dictyostelium Discoideum

Final concentration is 40mM Tris Base

Tryptone 10 NaCl 8 Agar 15 Final pH 7

0025 Magnesium sulfate 0

in the 1960s

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron Size:1000g Improvement of a synthetic mediumBoric acid, HBO3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Boron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room