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Autoclaves for sterilization in laboratories - Guide to safe use and operation Ingestion-build-73073 The split-beam method has been1 Scope This Part of BS 2646 gives guidance on the factors that should be taken into account when devising procedures to ensure the safe and effective use of laboratory autoclaves of the types specified in BS 2646 1, i. e. autoclaves for the sterilization of material and equipment including those which may be contaminated with organisms categorized as Hazard Groups 1, 2 or 3 (see note). It does not cover the use of autoclaves for material contaminated