Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium Size:1000g The antibiotic is inactivated by

The antibiotic is inactivated by means of incorporation of the APH 4 gene

Glycerol  252

The antibiotic binds reversibly to the 50S subunit of the bacterial ribosome

Agar has been omitted from the original formulation

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium Size:1000g The antibiotic is inactivated byMagnesium sulphate, MgSO4. 7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 2 gram powdered medium in 1 litre distilled water Store