Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g Tryptone 12 Yeast Extract 24

Tryptone 12 Yeast Extract 24 KH2PO4 9

MOPS is frequently used as a buffering agent in biology and biochemistry

coli strains are provided by LB Broth

Chemical formula C4H8N2O3H2O Solubility (20° C) 22 g/l Mol

Amongst all amino acids especially Tryptophan is present in high concentrations

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g Tryptone 12 Yeast Extract 24Manganese sulphate, MnSO4. H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store